Journal: Molecules

Article Title: Exploring EGFR, Nectin-4, and TROP-2 as Therapeutic Targets for Bladder Cancer Photoimmunotherapy

doi: 10.3390/molecules30244802

Figure Lengend Snippet: ( a ) Western blot analysis of EGFR, Nectin-4, and TROP-2 expression in the BC cell lines RT4 and RT112 and in the antigen-negative control cell line CHO. ( b ) Cell binding of the antibodies and conjugates to the target cells as demonstrated by flow cytometry. Binding of the isotype control antibody is shown in gray.

Article Snippet: RT4 cells were cultivated in EMEM medium (Cytion, Eppelheim, Germany), RT112 cells in RPMI1640 medium (Gibco), and CHO cells in F-12 Nutrient Mixture Medium (Gibco) at 37 °C and 5% CO 2 .

Techniques: Western Blot, Expressing, Negative Control, Binding Assay, Flow Cytometry, Control

Journal: Molecules

Article Title: Exploring EGFR, Nectin-4, and TROP-2 as Therapeutic Targets for Bladder Cancer Photoimmunotherapy

doi: 10.3390/molecules30244802

Figure Lengend Snippet: Cytotoxicity of the conjugates Cmb T120C/D265C -WB692-CB2, Enf T120C/D265C -WB692-CB2, and Sac T120C/D265C -WB692-CB2 after irradiation with different light doses in RT4 and RT112 cells. CHO cells served as antigen-negative controls. Mean values ± SD of three independent biological experiments. Statistical analyses were performed using unpaired, parametric Student’s t -tests with Welch’s correction (* p < 0.05, ** p < 0.01, *** p < 0.005, **** p < 0.001).

Article Snippet: RT4 cells were cultivated in EMEM medium (Cytion, Eppelheim, Germany), RT112 cells in RPMI1640 medium (Gibco), and CHO cells in F-12 Nutrient Mixture Medium (Gibco) at 37 °C and 5% CO 2 .

Techniques: Irradiation

Journal: Molecules

Article Title: Exploring EGFR, Nectin-4, and TROP-2 as Therapeutic Targets for Bladder Cancer Photoimmunotherapy

doi: 10.3390/molecules30244802

Figure Lengend Snippet: Cytotoxicity of the conjugates Cmb T120C/D265C -WB692-CB2, Enf T120C/D265C -WB692-CB2 and Sac 120T/265C -WB692-CB2 alone and in combination after irradiation with a light dose of 64 J/cm 2 in RT4 and RT112 cells. Control samples were treated with the uncoupled antibodies or the free dye. Mean values ± SD of three independent biological experiments. Statistical analyses were performed using unpaired, parametric Student’s t -tests with Welch’s correction (* p < 0.05, ** p < 0.01, *** p < 0.005, **** p < 0.001).

Article Snippet: RT4 cells were cultivated in EMEM medium (Cytion, Eppelheim, Germany), RT112 cells in RPMI1640 medium (Gibco), and CHO cells in F-12 Nutrient Mixture Medium (Gibco) at 37 °C and 5% CO 2 .

Techniques: Irradiation, Control

Journal: Oncogene

Article Title: Epigenomic mapping identifies an enhancer repertoire that regulates cell identity in bladder cancer through distinct transcription factor networks

doi: 10.1038/s41388-023-02662-1

Figure Lengend Snippet: A Cell viability in RT112 and SCaBER under siRNA treatment against FOXA1. B Venn diagram comparing differentially expressed genes in RT112 and SCaBER FOXA1 KD. C GSEA plot of Msig Hallmark GSEA Analysis of genes differentially regulated in RT112 and SCaBER cell lines upon FOXA1 siRNA (2 independent siRNA, 2 replicates). D Heatmap of genes in Hallmark interferon gamma response genes that are differentially regulated in FOXA1 KD vs Ct (min Fold Change = 1,5). E Heatmap of Top Luminal TFs expression in RT112 and SCaBER cell lines upon FOXA1 KD. F PCA projection of TCGA Tumours and CRispR mutant clones on the Basal/Luminal signatures. G GSVA analysis of FOXA1 CRispR mutant clones on Urothelial differentiation signature from Eriksson et al. H GSVA analysis of FOXA1 CRispR mutant clones on Basal TFs identified in Fig. I Overrepresentation analysis of DEG in FOXA1 mutant vs Controls. J Volcano plot of Deseq2 RNA-seq analysis comparing pooled CRispR mutant FOXA1 clones in SD48 and RT112 versus controls. K Transient overexpression of HA-FOXA1 in mutant FOXA1 CRispR clones, wildtype RT112 and SCaBER. qPCR expression of ZBED2 after transfection of HA-FOXA1 relative to control plasmid, 4 days post transfection including 24 h of Puromycin selection ( n = 3 for CrispR clones, n = 2 for WT RT112 and SCaBER). Significance was calculated using 2way ANOVA test ( p -value < 0.05 = *).

Article Snippet: The human bladder cancer-derived cell lines RT112, 5637, KK47, and SCaBER were obtained from DSMZ (Heidelberg, Germany).

Techniques: Expressing, CRISPR, Mutagenesis, Clone Assay, RNA Sequencing Assay, Over Expression, Transfection, Plasmid Preparation, Selection

Journal: Oncogene

Article Title: Epigenomic mapping identifies an enhancer repertoire that regulates cell identity in bladder cancer through distinct transcription factor networks

doi: 10.1038/s41388-023-02662-1

Figure Lengend Snippet: A TCGA expression of ZBED2 by Subtypes. B TCGA expression Heatmap of ZBED2 and FOXA1 and TCGA correlation between ZBED2 and FOXA1. C Expression of FOXA1 and ZBED2 in single-cell transcriptomics from bladder cancer cell lines in the Cancer Cell Line Encyclopedia (CCLE), highlighting the nearly mutually exclusive expression of these genes. D Genome browser view of ZBED2 and FOXA1 loci in SD48 and 5637 cell lines. E GSEA analysis (Hallmark) of ZBED2 correlated genes in basal cells population of GSM4307111 scRNA-seq Tumour. F GSEA analysis (Hallmark) of gene expression upon siZBED2 KD in RT112 (siZBED2-1 and siZBED2-2). G 3’seq STAT2 and CD274 (PD-L1) expression in RT112 and SCaBER after siZBED2 and siFOXA1.

Article Snippet: The human bladder cancer-derived cell lines RT112, 5637, KK47, and SCaBER were obtained from DSMZ (Heidelberg, Germany).

Techniques: Expressing, Single-cell Transcriptomics